Effects of mechanical stress on the formation and expression of Cbfalpha1 in vitro
Wang, X.-j.; Hao, X.-n.; Liu, W.; Peng, W.; Xu, Y.-h.
Hua Xi Kou Qiang Yi Xue Za Zhi 26(6): 588-590; 598
2008
ISSN/ISBN: 1000-1182 PMID: 19186847 Document Number: 624835
To evaluate the effects of mechanical stress on the formation and expression of core binding factor alpha1 (Cbfalpha1) in MG-63 cells cultured on titanium in vitro. MG-63 cells cultured on the titanium were subjected to a centrifugal force (2.205 N) 15 min per 4 hours and collected after 4, 8 and 12 hours. The formation and expression of Cbfalpha1 were examined by immunofluorescence staining and reverse transcription-polymerase chain reaction (RT-PCR). Both the cells with or without centrifugal force created the fluorescence in the nucleus and the immunofluorescence intensity of Cbfalpha1 in MG-63 cells with centrifugal force were higher than those without centrifugal force (P<0.05). Meanwhile, both the cells with or without centrifugal force expressed the mRNA of Cbfalpha1 and the relative mRNA level of Cbfalpha1 in MG-63 cells with centrifugal force were higher than those without centrifugal force, and the differences were great significant (P<0.05). Mechanical stress are beneficial to the formation and expression of Cbfalpha1.