Determination of 2-methoxy-3,6-dichlorobenzoic acid (Banvel D) in apples by thin-layer chromatography
Vaĭntraub, F.P.; Nesterova, I.P.
Gigiena i Sanitariia 38(5): 79-81
1973
ISSN/ISBN: 0016-9900 PMID: 4769803 Document Number: 62376
A method is described for the extraction and determination by t.l.c. of Banvel-D (dicamba) residues in apples. The residue is extracted from homogenized apples with water. The solution is filtered, combined with sodium hydroxide and treated with thioether to remove wax and pigments. The dimethylamine salt is subsequently converted to the free acid. Dicamba is finally extracted with petroleum ether, the extract distilled and the dry residue dissolved in thioether prior to application to a silica layer. Ethyl acetate with cyclohexane, benzene with chloroform, dioxane and formic acid and thioether with petroleum ether and formic acid are used as solvent systems. Spots are visualized with ammoniacal silver nitrate. Dicamba appears in the form of brown spots under u.v. light. The sensitivity of the method is 0.4 mg/kg.