Constitutive expression of IL-7 receptor alpha does not support increased expansion or prevent contraction of antigen-specific CD4 or CD8 T cells following Listeria monocytogenes infection
Haring, J.S.; Jing, X.; Bollenbacher-Reilley, J.; Xue, H-Hui.; Leonard, W.J.; Harty, J.T.
Journal of Immunology 180(5): 2855-2862
2008
ISSN/ISBN: 0022-1767 PMID: 18292507 Document Number: 620703
Expression of IL-7R alpha (CD127) has been suggested as a major determinant in the survival of memory T cell precursors. We investigated whether constitutive expression of IL-7R alpha on T cells increased expansion and/or decreased contraction of endogenous Ag-specific CD4 and CD8 T cells following infection with Listeria monocytogenes. The results indicate that constitutive expression of IL-7Ra alone was not enough to impart an expansion or survival advantage to CD8 T cells responding to infection, and did not increase memory CD8 T cell numbers over those observed in wild-type controls. Constitutive expression of IL-7R alpha did allow for slightly prolonged expansion of Ag-specific CD4 T cells; however, it did not alter the contraction phase or protect against the waning of memory T cell numbers at later times after infection. Memory CD4 and CD8 T cells generated in IL-7R alpha transgenic mice expanded similarly to wild-type T cells after secondary infection, and immunized IL-7Ra transgenic mice were fully protected against lethal bacterial challenge demonstrating that constitutive expression of IL-7R alpha does not impair, or markedly improve memory/secondary effect-or T cell function. These results indicate that expression of IL-7R alpha alone does not support increased survival of effector Ag-specific CD4 or CD8 T cells into the memory phase following bacterial infection.