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PrP protein can bind to protein kinase CK2 both in native and recombinant forms in vitro

Chen, J-Ming.; Gao, C.; Shi, Q.; Gao, Y-Jun.; Lei, Y-Jun.; Shan, B.; Dong, C-Fang.; Wang, G-Rong.; Shi, S.; Han, J.; Dong, X-Ping.

Bing du Xue Bao 24(5): 335-339

2008

ISSN/ISBN: 1000-8721
PMID: 19035320
Document Number: 619776
The full length sequences of human CK2- alpha and CK2- beta were amplified with RT-PCR using mRNA from cell line SH-SY5Y as template, and the fusion proteins HIS-CK2- alpha and GST-HIS-CK2- beta were expressed in Escherichia coli to determine the molecular interaction between CK2 and PrP. Results revealed that recombinant PrP bound specifically with CK2- alpha , but not with CK2- beta. The native CK2 and PrP in the hamster brains interacted and formed protein complexes.

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