Involvement of EGF receptor and PI3K in ICAM-1 expression in human airway epithelial cells exposed to zinc sulfate
Zhang, Q.; Wu, W.; Zhang, T.; Shi, S.
Wei Sheng Yan Jiu 37(5): 552
2008
ISSN/ISBN: 1000-8020 PMID: 19069650 Document Number: 619433
To explore the effect of zinc sulfate on the expression of intercellular adhension molecule 1 (ICAM-1) and underlying mechanisms. A human bronchial epithelial cell line (BEAS-2B) was treated with zinc sulfate. The expression of ICAM-1 mRNA and protein and activation of epithelial growth factor receptor (EGFR) and PI3K was determined using PCR and immunoblotting. Zinc sulfate stimulation induced overexpression of ICAM-1 mRNA and protein. Under the same experimental condition, exposure to zinc sulfate increased phosphorylatihon of EGFR and Akt, the downstream kinase of PI3K. Pretreatment of BEAS-2B cells with the EGFR inhibitor PD153035 and the PI3K inhibitor LY294002 significantly blocked zinc sulfate-induced ICAM-1 expression, Zinc sulfate stimulation could activate the EGFR and the PI3K/Akt signaling pathway, further leading to upregulation of ICAM-1 expression.