Construction of CyclinD (-/-) /Patched (+/-) double knockout mice
Xin, S.-J.; Li, X.; Molly, D.-S.; Wei, D.
Zhonghua Yi Xue Za Zhi 88(3): 185-188
2008
ISSN/ISBN: 0376-2491 PMID: 18361818 Document Number: 617709
To construct CyclinD (CycD) (-/-)/Patched (Ptc) (+/-) double knockout mice to study the relationship between Hedgehog and Rb signaling pathways. Amphimixis was performed between female heterozygote CycD knockout mice CycD (+/-) and male heterozygote Ptc knockout mice Ptc (+/-), thus CycD (+/-)/Ptc (+/-) double knockout mice were obtained. These mice underwent secondary matched and thus construct CycD (-/-)/Ptc (+/-) double knockout mice. CycD (-/-)/Ptc (+/-) double knockout mice, CycD (+/-)/Ptc (+/-) heterozygote double knockout mice, and CycD knockout mice CycD (-/-)/Ptc (+/+) were gained. The method of amphimixis can construct CycD (--)/Ptc (+/-) double knockout mice that can be used to disclose the regulation between Ptc gene and CycD gene and the relativity between Hedgehog and Rb pathways in mammals.