Electron cryotomography
Murphy, G.E.; Jensen, G.J.
Biotechniques 43(4): 413 415 417 Passim
2007
ISSN/ISBN: 0736-6205 PMID: 18019332 Document Number: 614453
Electron cryotomography is an emerging technique that allows the structures of unique biological objects such as individual macromolecules, viruses, and even small whole cells to be reconstructed in their near-native states in three dimensions (3-D) to an approximate 5-nm resolution. The required instrumentation, sample preparation and limitations, data collection, typical results, and future prospects are summarized briefly.
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