Study on mechanism of abnormal savda munziq flavonoids in induction of apoptosis of Hep2 cells
Yusup, A.; Upur, H.; Tursun, X.; Berke, B.én.éd.; Baudrimont, I.; Moore, N.
Zhongguo Zhong Yao Za Zhi 32(11): 1068-1071
2007
ISSN/ISBN: 1001-5302 PMID: 17672346 Document Number: 613275
To investigate the effect of Abnormal Savda Munziq (ASMq) flavonoids on proliferation, apoptosis and apoptosis-related gene expression in human hepatoma (HepG2) cells in vitro and to probe the mechanism. The effects of ASMq flavonoids on proliferation, apoptosis and apoptosis-related gene expression of HepG2 cells were investigated respectively by MTT assay, gel electrophoresis, flow cytometry and RT-PCR. ASMq flavonoids significantly inhibited growth of HepG2 cells in vitro, arrested HepG2 in the sub-G, phase, induced cell apoptosis and significantly down-regulated expression level of Bcl-2 mRNA, and up-regulated expression of p53, p21, Bax gene mRNA expressions. ASMq flavonoids has significantly regulative action on growth, apoptosis and apoptosis-related gene expression of cancer cells in vitro, which possibly are the important way to excert anticancer effect, and flavonoids are possibly a main active component of ASMq for exerting the anticancer effect.