Expression of annexin i in human pancreatic cancer and the influence of its down-regulation on biology of this cancer
Liu, Q.; Wang, C.-F.; Zhou, Z.; Hu, H.; Zhao, D.-B.; Ni, X.-G.; Bai, X.-F.; Gao, J.-D.; Tian, Y.-T.; Zhao, P.
Zhonghua Zhong Liu Za Zhi 29(10): 738-741
2007
ISSN/ISBN: 0253-3766 PMID: 18396684 Document Number: 613119
To investigate the expression of annexin in human pancreatic cancer and to elucidate its role in oncogenesis of pancreatic cancer. A pancreatic carcinoma cell line Suit-II with high-expression of annexin I gene was adopted. Three subtypes of annexin I -siRNA sequences and a non-related fragment were combined, and the eukaryotic expression vectors bearing siRNA fragments were constructed. Then they were transfected into pancreatic carcinoma cells to knock down the expression of annexin I by RNAi. After knocking down the expression of annexin I , the growth speed, cell cycling, morphological features and apoptosis of pancreatic carcinoma cells were examined by RT-PCR and MTT test. When the expression of annexin I was blocked, the growth speed of pancreatic carcinoma cells was significantly decreased, the morphological features were changed and pronounced apoptosis occurred. Annexin I can modulate pancreatic carcinoma cell cycle, promote the cell proliferation, increasingly stimulate the cell growth, and suppress the process of apoptosis in pancreatic carcinoma cells.