Top Abstract Purchase Related
Home
>
Node
>
Subnode

Sensitive detection of Pneumocystis jirovecii DNA in gastric wash using nested polymerase chain reaction

Limawongpranee, S.; Wanachiwanawin, D.; Chokephaibulkit, K.; Onchrotchanakun, J.; Lertlaituan, P.; Wankhom, S.

Southeast Asian Journal of Tropical Medicine and Public Health 38(5): 892-896

2007

ISSN/ISBN: 0125-1562
PMID: 18041308
Document Number: 612674
One hundred and five samples of gastric washes were obtained from 52 pediatric patients. Eleven of the 105 samples (10%) gave positive results using immunofluorescence antibody test (IFA) for Pneumocystis firovecii. Single-step polymerase chain reaction (PCR) produced 13 % (14 samples), whereas detection by nested PCR was increased to 65 samples (62%). Moderate agreement (kappa = 0.5) was found between test results of IFA and single-step PCR, but no agreement was found between the results of IFA and nested PCR (kappa = 0.1).

Document emailed within 1 workday
Secure & encrypted payments

Ottiwet, O.; Chomvarin, C.; Chaicumpar, K.; Namwat, W.; Mairiang, P. 2010: Nested polymerase chain reaction for detection of Helicobacter pylori in gastric biopsy specimens Southeast Asian Journal of Tropical Medicine and Public Health 41(6): 1423-1431
Gupta, R.; Mirdha, B.Ranjan.; Guleria, R.; Mohan, A.; Kabra, S.Kumar.; Kumar, L.; Agarwal, S.Kumar.; Luthra, K. 2008: Use of different primer directed sequence amplification by polymerase chain reaction for identification of Pneumocystis jirovecii in clinical samples Indian Journal of Chest Diseases and Allied Sciences 50(4): 321-327
Yamashita, K.; Otsuka, N.; Nakadoori, Y.; Kagawa, S.; Matsuoka, A. 1995: Detection of human cytomegalovirus DNA by nested polymerase chain reaction Rinsho Byori. Japanese Journal of Clinical Pathology 43(1): 67-73
Vythilingam, I.; Nitiavathy, K.; Yi, P.; Bakotee, B.; Hugo, B.; Singh, B.; Wirtz, R.A.; Palmer, K. 1999: A highly sensitive, nested polymerase chain reaction based method using simple dna extraction to detect malaria sporozoites in mosquitos Southeast Asian Journal of Tropical Medicine and Public Health 30(4): 631-635
Song, M. 1993: Detection of Helicobacter pylori in human saliva by using nested polymerase chain reaction Zhonghua Liu Xing Bing Xue Za Zhi 14(4): 237-240
Arai, M.; Mizukoshi, C.; Kubochi, F.; Kakutani, T.; Wataya, Y. 1994: Detection of Plasmodium falciparum in human blood by a nested polymerase chain reaction American Journal of Tropical Medicine and Hygiene 51(5): 617-626
Zhu, X.; Zhuang, H.; Zhu, W. 1996: Establishment and application of reverse transcription-nested polymerase chain reaction for detection of hepatitis E RNA in serum Zhonghua Yu Fang Yi Xue Za Zhi 30(6): 334-336
Qiao, F.-y.; Huang, Y.-m.; Li, Z.-q.; Liu, H.-y.; Tang, H.-j. 2004: Detection of human parvovirus B19 nonstructural protein DNA by nested- polymerase chain reaction in pregnant women Zhonghua Fu Chan Ke Za Zhi 39(2): 86-90
Shang, S.; Hong, W.; Shi, Y. 1998: Detection of human cytomegalovirus infection in pregnant women and their fetuses by nested polymerase chain reaction Zhonghua Fu Chan Ke Za Zhi 33(3): 136-138
Honda, K.; Nakatome, M.; Islam, M.N.; Bai, H.; Ogura, Y.; Kuroki, H.; Yamazaki, M.; Terada, M.; Misawa, S.; Wakasugi, C. 1995: Detection of D1S80 (pMCT118) locus polymorphism using semi-nested polymerase chain reaction in skeletal remains Journal of Forensic Sciences 40(4): 637-640
Cathomas, G.; McGandy, C.; Diamantis, I.; Tichelli, A.; Gudat, F. 1994: Detection of bcr-abl transcripts in "minimal residual disease" in chronic myelogenous leukemia by nested polymerase chain reaction (PCR) Verhandlungen der Deutschen Gesellschaft für Pathologie 78: 314-317
Cannone, M.; Vago, L.; Porini, G.; Bonetto, S.; Cassi, C.; Bramerio, M.; Rizzato, G.; Barberis, M.C. 1997: Detection of mycobacterium tuberculosis DNA using nested polymerase chain reaction in lymph nodes with sarcoidosis, fixed in formalin and embedded in paraffin Pathologica 89(5): 512-516
Lin, J.C.; Tsai, C.S.; Wang, W.Y.; Jan, J.S. 2000: Detection of circulating tumor cells in venous blood of nasopharyngeal carcinoma patients by nested reverse transcriptase-polymerase chain reaction Kaohsiung Journal of Medical Sciences 16(1): 1-8
Klincheva, S.A.; Bakaev, V.V.; Barinskiĭ, I.F.; Gnedoĭ, S.N.; Nikolaeva, N.P. 1996: The use of nested polymerase chain reaction method for rapid detection of cytomegalovirus in peripheral blood leukocytes of patients after bone marrow transplantation Voprosy Virusologii 41(4): 147-149
Hioki, T.; Sugimura, Y. 1999: Detection of circulating cancer cells by nested reverse transcription-polymerase chain reaction of cytokeratin-19 in patients with renal cell carcinoma Hinyokika Kiyo. Acta Urologica Japonica 45(8): 577-581
Tani, N.; Ichikawa, D.; Ikoma, D.; Ikoma, H.; Sai, S.; Tomita, H.; Okamoto, K.; Kikuchi, S.; Fujiwara, H.; Ochiai, T.; Ueda, Y.; Otsuji, E.; Hagiwara, A.; Yamagishi, H. 2006: An early detection of recurrence using reverse transcriptase-polymerase chain reaction (RT-PCR) and methylation-specific polymerase chain reaction (MSP) from peripheral blood in patients after gastrectomy Gan to Kagaku Ryoho. Cancer and ChemoTherapy 33(12): 1720-1722
Kim, O.; Kim, S.S. 2003: A sensitive and specific detection of Human herpesvirus 8 by polymerase chain reaction and dot blot hybridization Acta Virologica 47(2): 87-90
Zhong, X.Y.; Lin, Y.S.; Kaul, S.; Bastert, G. 2000: Sensitive and Specific Detection of Carcinoembryonic Antigen cDNA Using the Hot Start Polymerase Chain Reaction Technique Clinical Laboratory 46(1-2): 7-11
Ye, R.; Pan, J-cao.; Huang, Z-cheng.; Wang, H.; Wang, H-qiu.; Wei, D-fang.; Xu, K.; Wen, H-gen.; Chen, K-kai. 2005: Detection of severe acute respiratory syndrome probable patients' virus RNA in Hangzhou by using a two loci and a modified nested real-time reverse transcription polymerase chain reaction Zhonghua Yu Fang Yi Xue Za Zhi 39(2): 129-132
Zhou, F.; Cao, X.; Liu, M.; Wang, Y.; Tao, G. 2011: A Study of the Methylation Status of Opioid Binding Protein / Cell Adhesion Molecule-Like Gene in Ovarian Cancer Using Nested Methylation-Specific Polymerase Chain Reaction Detection Clinical Laboratory 57(5-6): 421-424