JAK3 mRNA level in graft versus host disease patients: a preliminary study
Li, Q.-h.; Du, X.; Geng, S.-x.; Weng, J.-y.; Guo, R.; Lu, Z.-s.; Lin, W.; Huang, Z.-l.
Zhonghua Yi Xue Za Zhi 87(28): 1981-1983
2007
ISSN/ISBN: 0376-2491 PMID: 17923039 Document Number: 612498
To quantify peripheral blood mononuclear cell JAK3 mRNA levels after allogeneic haematopoietic stem cell (allo-HSCT) transplantation, and to explore its relationship with graft vs host disease (GVHD). Serial monitoring of JAK3 mRNA levels by fluorescent quantitative reverse transcriptase polymerase chain reaction (FQ-RT-PCR) technique was performed on 26 cases (83 samples) after allo-HSCT. The mean JAK3/ABL expression levels was 8.71 in 14 patients without GVHD; the JAK3/ABL expression level was 31.94 in one patient with acute GVHD (aGVHD); the mean JAK3/ABL expression levels was 19.23 in 11 patients with chronic GVHD (cGVHD), and the cGVHD was diagnosed at the mean time of six months (3 - 12 months). The JAK3/ABL expression levels did not change in 4 patients one month after allo-HSCT, however, the levels increased again when GVHD were diagnosed in these 4 patients, and the JAK3/ABL expression levels decreased again when GVHD was remitted. The JAK3 expression level related to the remission and relapse in patients with GVHD.