Methylation status of LINE-1 sequences in patients with MDS or secondary AML
Römermann, D.; Hasemeier, B.; Metzig, K.; Schlegelberger, B.; Länger, F.; Kreipe, H.; Lehmann, U.
Verhandlungen der Deutschen Gesellschaft für Pathologie 91: 338-342
2007
ISSN/ISBN: 0070-4113 PMID: 18314632 Document Number: 612418
This study analyzes changes in the degree of global methylationlevel in myelodysplastic syndrome during progression of the disease. Methylation status was analyzed in 127 patients with histologically confirmed MDS and 26 reactive controls. We employed Pyrosequencing, Luminometric Methylation Assay (LUMA) and a realtime PCR-based quantitative assay. We detected an increase of methylation level of LINE-1 sequences using pyrosequencing and an increase of methylation in the HpaII recognition site employing LUMA during the progression of MDS. Methylation sensitive quantitative PCR showed no statistically significant differences, only a trend. LINE-1 and methylation sensitive cleavage of DNA can act as a surrogatmarker for global DNA methylation. The genome wide hypermethylation of MDS is a distinct feature of this disease. It discriminates MDS from other neoplasia and may explains the success of hypomethylation inducing reagents like azadeoxycytidine in MDS therapy.