Effect of TLR4 on expressions of p-IRAK1 and p-IkappaB-alpha in HPDLCs stimulated by lipopolysaccharide
Sun, Y.; Shu, R.; Zhang, M.-z.; Li, C.-l.
Shanghai Kou Qiang Yi Xue 16(6): 632-635
2007
ISSN/ISBN: 1006-7248 PMID: 18278418 Document Number: 611399
To evaluate the effect of TLR4 on expressions of p-IRAK1 and p-IkappaB-alpha in HPDLCs stimulated by lipopolysaccharide. Western blot and image analysis were used to detect the levels of p-IRAK1 and p-IkappaB-alpha in HPDLCs stimulated by 1 microg/ml LPS after 2.5,5,10 and 15 minutes, and to detect the levels of these proteins in HPDLCs stimulated by 1 microg/ml LPS after pretreatment with monoclonal antibody of TLR4. Statistical analysis was performed with SPSS10.0 software package for one-way ANOVA. The expressions of p-IRAK1 and p-IkappaB-alpha in HPDLCs were enhanced after stimulation of LPS. The gray scale values of protein band at 0 minute were 0.054, 0.19 respectively, significantly increased to 0.785, 0.809 after 5 minutes (P<0.05). After pretreatment with monoclonal antibody of TLR4, the expressions of these proteins weakened compared with those without pretreatment. The gray scale values of protein band significantly decreased from 0.82,0.874 to 0.099,0.201, respectively (P<0.05). TLR4 is involved in the signaling transduction in HPDLCs stimulated with LPS, and might be involved in the progression of periodontis.