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In vitro gene transfer using cationic vectors, electroporation and their combination

Weecharangsan, W.; Opanasopit, P.; Lee, R.J.

Anticancer Research 27(1a): 309-313

2007

ISSN/ISBN: 0250-7005
PMID: 17354325
Document Number: 610046
Background: The aim of this study was to investigate the transfection efficiency of cationic vectors (polyethylenimine; PEI25K and lipofectamine (TM)), electroporation and their combination in the human cancer cell lines, Raji human lymphoma and KB human oral carcinoma. Materials and Methods: Raji human lymphoma and KB human oral carcinoma cell lines were transfected with pcDNA3-CMV-Luc at various NIP ratios of cationic vectors and voltages of electroporation, as well as with a combination of the cationic vectors and electroporation. Results: The major findings were: (a) cationic vectors or electroporation alone increased transfection efficiency; (b) cationic vectors inhibited the transfection efficiency by electroporation. Conclusion: Our results demonstrate that cationic vectors and electroporation are feasible and efficient in transfecting human cancer cell lines. However, a combination of cationic vectors and electroporation is ineffective.

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