Molecular genetic methods of typing of the Bartonellae
Kirillov, M.Iu.; Markov, A.P.; Lopyrev, I.V.; Pankratova, V.N.; Levitskiĭ, S.A.; Bashkirov, V.N.; Smirnov, G.B.; Kruglov, A.N.; Osadchaia, V.A.; Frolova, G.P.; Barmina, G.V.; Morozova, O.A.; Kosoy, M.Y.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 2007(1): 8-15
2007
ISSN/ISBN: 0208-0613 PMID: 17354603 Document Number: 608946
The primer systems for the PCR detection of four house-keeping genes of bartonellae in clinical material were developed and tested. The tactics of the species RFLP typing was also developed and tested. The scheme of the species RFLP typing of bartonellae was tested using as an example two strains for the first time isolated in Russia from patients with endocarditis and fever of uncertain origin. The results of the typing were supported by sequencing of the amplicons obtained. According to the sequencing the isolates were attributed to the sub species Bartonella vinsonii, subsp. arupensis. The necessity of molecular epidemiological analysis of bartonelloses in Russia was substantiated.