Changes in the expression of voltage-dependent ca2+ channels in asthenospermia
Li, L.; Liu, J-hong.
Zhonghua Nan Ke Xue 13(8): 706-709
2007
ISSN/ISBN: 1009-3591 PMID: 17918709 Document Number: 608525
To study the relationship between the expression of voltage-dependent Ca2+ channels (VDCCs) cal mRNA and asthenospermia. Based on the WHO criteria, we filtered the donated semen by computer-aided sperm analysis (CASA), optimized the ejaculated sperm by discontinuous Percoll grads centrifugation, and examined and quantitated the multitype VDCCs alpha1 mRNA expressions in the sperm of normal men and asthenospermia patients using reverse transcription polymerase chain reaction (RT-PCR). Compared with the normal sperm, there were significant differences among the expressions of alpha1B (39.40 +/- 9.47), alpha1C (48.30 +/- 11.60), alpha1E (3.20 +/- 0.78), alpha1G (8.40 +/- 2.03) and alpha1H (5.70 +/- 1.47) mRNA messages in asthenospermia (P < 0.01). The abnormal expression of L-type and/or non-L-type VDCCs' mRNAs may be one of the causes of asthenospermia.