Association between the +491C/T polymorphism of beta2-adrenergic receptor gene and risk of essential hypertension in Kazakns of Xinjiang
Li, N-fang.; Zhou, L.; Li, T.; Wang, H-mei.; Liu, H.; Hu, J-li.
Zhonghua Xin Xue Guan Bing Za Zhi 35(8): 706-709
2007
ISSN/ISBN: 0253-3758 PMID: 17963627 Document Number: 607328
Objective To investigated the association between the +491C/T polymorphism of AR gene and risk of essential hypertension (EH) in Kazakns of Xinjiang. Methods In this population-based case-control study, we recruited 507 Kazakns subjects (289 EH and 218 normotensives, aged from 3060 years old) from the pastoral areas in Xinjiang. Peripheral blood were collected and the DNA was extracted by means of a standard phenol-chloroform method. The + 491 C/T genotypes of beta(2)-AR was identified by PCR-restriction fragment length polymorphism analysis. The distribution of genotypic and allelic frequencies between EH and controls were also compared. Results The frequencies of the genotype CC, CT were 98.82% and 1.18%, respectively and there was no TT genotype in this population, the genotype distribution was consistent with Hardy-Weinberg equilibrium (chi(2) =0.018, P =0.893). CC and CT genotypic frequencies were 97.92% and 2.08% respectively in EH, while there was no CT genotype in normotensives. The allelic frequencies of C, T were significantly different between EH (98.96% 1.04%) and normotensives ( 100% 0%, all P = 0. 040). Systolic blood pressure and diastolic blood pressure were similar in subjects with various genotypes in the whole population ( P > 0. 05) but systolic blood pressure was significantly higher in males with CT genotype compared with males with CC genotype ( P = 0. 046). Conclusion The +491C/T polymorphism of beta(2)-AR gene is associated with essential hypertension and is a possible susceptible factor for essential hypertension in Kazakns of Xinjiang.