Development of recombinant scFv-antibodies against diphtheria toxin using phage display system

Oleĭnik, E.S.; Kaberniuk, A.A.; Burkaleva, D.A.; Romaniuk, S.I.; Kolibo, D.V.; Shepeliakovskaia, A.O.; Laman, A.G.; Komisarenko, S.V.; Oleinik, E.S; Kaberniuk, A.A; Burkaleva, D.A; Romaniuk, S.I; Kolibo, D.V; Shepeliakovskaia, A.O; Laman, A.G; Komisarenko, S.V

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Development of recombinant scFv-antibodies against diphtheria toxin using phage display system

Oleĭnik, E.S.; Kaberniuk, A.A.; Burkaleva, D.A.; Romaniuk, S.I.; Kolibo, D.V.; Shepeliakovskaia, A.O.; Laman, A.G.; Komisarenko, S.V.

Ukrains'kyi Biokhimichnyi Zhurnal 79(5): 91-97

2007

ISSN/ISBN: 0372-3909

PMID: 18357781

Document Number: 606187

Phage display technology is an effective approach to the development of the next generation of immunodiagnostic reagents. Naive murine phage display a library of single-chain variable antibodies (scFv) was used to isolate scFv recognizing the diphtheria toxin, an important diagnostic antigen of diphtheria. The diphtheria toxin B subunit-binding clone with affinity constant of 1.13 x 10(7) M(-1) was selected. scFv preserved activity on storage in the course of 8 months.