Study on the correlation between gene ponA/penA and the penicillin-resistance of Neisseria gonorrhoeae
Zhang, T.-J.; Zhou, X.-M.; Zhang, T.; Jiang, Q.-W.
Zhonghua Liu Xing Bing Xue Za Zhi 28(9): 901-905
2007
ISSN/ISBN: 0254-6450 PMID: 18251277 Document Number: 605831
To investigate the relationship between penA/ponA and penicillin resistance of Neisseria gonorrhoeae. Agar dilution method was used to determine the minimum inhibitory concentrations (MICs) of the strains. Polymerase chain reaction-single stand conformation polymerphism (PCR-SSCP) and PCR- restriction fragment length polymorphism (RFLP) were used to detect the mutations in ponA and penA genes, which encoding the penicillin binding protein-1 and -2 (PBP1 and PBP2), respectively. All the 80 N. gonorrhoeae isolates had a D345 insertion detected in penA while 93.7% of N. gonorrhoeae isolates having a point mutation Leu421 --> Pro in ponA. Most of the penicillinase producing N. gonorrhoeae (PPNG) strains possessed the mutations in ponA and penA. Our data suggested that the plasmid and chromosome mediated penicillin-resistance conjugately increased the level of resistance.