Effect of ginkgolide B on the platelet-activating factor induced changes of chemotaxis and cytoskeleton of macrophages
Peng, S-ying.; Zhang, F-ying.; Ou-Yang, X-yu.; Liu, Y.; Wang, W-jie.
Yao Xue Xue Bao 41(2): 156-160
2006
ISSN/ISBN: 0513-4870 PMID: 16671547 Document Number: 604917
Aim: To study the inhibitory effect of ginkgolide B (BN52021) on the platelet activating factor (PAF)-induced changes of chemotaxis of murine peritoneal macrophages and the related polymerization of F-actin. Methods: Chemotaxis assays were performed using a modified 48-well Boyden chamber. Actin polymerization of murine peritoneal macrophages was analysed by flow cytometry using a specific fluorescent stain. Results: Peritoneal macrophages significantly migrated toward PAF through a micropore filter; however, in the presence of PAF receptor antagonist BN52021 (0.01 nmol litre-1 -0.1 micro mol litre-1), the migration was significantly inhibited. Moreover, BN52021 inhibited the actin polymerization of murine peritoneal macrophages induced by PAF in the presence of Ca2+, but not in Ca2+-free medium. Conclusion: The results suggested that preventing polymerization of F-actin may be a pathway by BN52021 to inhibit the chemotaxis of macrophages, and this effect seems to be Ca2+-dependent. The data further indicated that inhibition of PAF induced macrophage chemotaxis is an important mechanism underlying the antiinflammatory action of BN52021.