Applications of real-time PCR technology to mycobacterial research
Parashar, D.; Chauhan, D.S.; Sharma, V.D.; Katoch, V.M.
Indian Journal of Medical Research 124(4): 385-398
2006
ISSN/ISBN: 0019-5340 PMID: 17159258 Document Number: 604306
Real-time polymerase chain reaction (PCR) is being used worldwide for various research purposes. In this review we discuss the principles of real-time PCR, different methodologies and chemistries available and their applications in mycobacterial research. This technology allows for the direct detection of PCR product during the exponential phase of the reaction. Being a very powerful, accurate, rapid and sensitive method, this technique holds immense promise for detection of mycobacteria, differentiation of mycobacterial species, quantification of mycobacterial load and detection of drug resistance in mycobacterial infection.