Quantitative study of osteoclastic related factors in the process of bone reconstruction
Chang, X.; Hou, Z.-M.; Sibata, Y.; Tsukazaki, T.; Yamaguchi, A.
Hua Xi Kou Qiang Yi Xue Za Zhi 24(2): 164-165; 169
2006
ISSN/ISBN: 1000-1182 PMID: 16704099 Document Number: 603628
To detect the expression of osteoclast related factors, tumor necrosis factor-alpha (TNF-alpha), receptor activator of NF-kappaB ligand (RANKL) and tartrate-resistant acid phosphatase (TRAP), in the process of bone remodeling. 8-week-old male C57BL/6J mice were employed in this study to detect the expression of osteoclast related factors by real time PCR. TNF-alpha, RANKL and TRAP were up regulated in the process of bone remodeling, they reached the peak on day 2, 5 and 10 individually after injury. Osteoclast related factors also participate in bone remodeling, which depends on the delicate balance between bone formation and bone resorption.