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Real-time polymerase chain reaction assay for detection of Streptococcus pneumoniae in sputum samples from patients with community-acquired pneumonia

Bayram, A.; Kocoglu, E.; Balci, I.; Filiz, A.; Eksi, F.

Journal of Microbiology Immunology and Infection 39(6): 452-457

2006

ISSN/ISBN: 1684-1182
PMID: 17164946
Document Number: 602196
Background and Purpose: A quantitative real-time polymerase chain reaction (PCR) method targeting the pneumolysin gene of Streptococcus pneumoniae in sputum specimens from patients with community-acquired pneumonia (CAP) was evaluated for the identification of pneumococci.Methods: The applicability of the assay to clinical samples was evaluated by studying 140 sputum specimens from patients with CAR Of the specimens, 96 (68.6%) were found to be positive by real-time PCR. The results were compared to culture findings.Results: The sensitivity and specificity of the real-time PCR assay developed in this study as compared to those of the culture method were 97.2% and 60.9%, respectively.Conclusion: Real-time PCR assay was found to be a rapid and sensitive method for the detection of pneumococci.

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