The comparison of mycoplasma detection methods in the urogenital tract infection
Balabanov, D.N.; Rakovskaia, I.V.; Gorina, L.G.; Goncharova, S.A.; Gamova, N.A.; Barkhatova, O.I.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2006(4): 82-85
2006
ISSN/ISBN: 0372-9311 PMID: 16941879 Document Number: 602099
Six different methods have been employed to detect M. hominis(Mh) and U. urealyticum (Uu) in clinical samples collected from 67 men. The results obtained by PCR and IF test were approximately equal: 13.6 and 13.44% - Mh and 44.4 and 48.8% - Uu, respectively. Mycoplasmas were detected by cultural method less frequently (9.6% - Mh, 32.2% - Uu). The highest infection rates were obtained in the test for blood antigens (40% - Mh and 63% - Uu). At present a commercial diagnosticum to detect mycoplasma antigents in blood is lacking. Sometimes the results of cultural method are positive, while the PCR results are negative. So the optimal scheme based on both PCR anf culture has been proposed.