Tension-force induced cyclooxygenase-2 expression mediated by microfilament in human periodontal ligament fibroblast
Xiong, P.-Y.; Huang, S.-G.; Zhang, J.-X.
Hua Xi Kou Qiang Yi Xue Za Zhi 24(4): 353-356
2006
ISSN/ISBN: 1000-1182 PMID: 16999359 Document Number: 596091
To study the role of microfilament polymerization in menchanotransduction by human periodontal ligament fibroblast (hPDLFs). In tension-force group, hPDLFs were treated by tension-force values of 18% for 8 h, 16 h, 24 h. In tension-force and inhibitor group, the sample was treated with 5 microg/mL cytochalasin B before using tension-forece. Each sample was collected and the expression of cyclooxygenase-2 was measured by using immunohistoche staining. In tension-force group, the expression of cyclooxygenase-2 enhanced with the extension of loading time. In tension-force and inhibitor group, cyclooxygenase-2 expression was depressed and had no relation with loading time. Tension-force induced cyclooxygenase-2 expression is mediated by microfilament, disruption of the microfilament polymerization will destroy mechanotransduction in hPDLFs.