Inhibition of caveolin-1 expression by incadronate in PC-3 prostate cells
Iguchi, K.; Matsunaga, S.; Nakano, T.; Usui, S.; Hirano, K.
Anticancer Research 26(4b): 2977-2981
2006
ISSN/ISBN: 0250-7005 PMID: 16886623 Document Number: 595091
Background: Caveolin-1 is an essential component of caveolae and its expression is known to be increased in human prostate cancer. The reduction of caveolin-1 expression has been reported to decrease the tumorigenic and metastatic potential of prostate cancer. Materials and Methods: Caveolin-1 expression was determined by real-time RT-PCR and Western blot analysis. Results: Incadronate, a third-generation bisphosphonate, was found to inhibit the caveolin-1 mRNA and protein expression in PC-3 prostate cells. The decrease in caveolin-1 mRNA expression by incadronate was prevented by co-incubation with geranylgeranyol, but not with farnesol. Moreover, treatment of GGTI-286, a geranylgeranyl transferase inhibitor, but not FTI-277, a famesyl transferase inhibitor, also resulted in the inhibition of caveolin-1 mRNA expression. Conclusion: These results indicate that the decrease in caveolin-1 expression elicited by incadronate is related to the inhibition of protein geranylgeranylation.