Establishment of reporter gene-based assays for the identification of (anti) androgenic effects of chemicals in CHO cells
Zhao, H-Tao.; Zhu, J-Bo.; Zhu, Y-Fei.; Wang, Z.; Ma, X.; Zhang, T.
Wei Sheng Yan Jiu 34(3): 281-284
2005
ISSN/ISBN: 1000-8020 PMID: 16111029 Document Number: 592144
Develop reporter gene-based assays for the identification of (anti) androgenic effects of chemicals in CHO cells, explore the antiandrogenic effects of 2,4-DDT and methoxychlor. Chinese Ovary cells were cotransfected with the human androgen receptor expression vector, mouse mammary tumour virus MMTV-luciferase vector and phRL-SV40 using the transfection reagent Sofast, test the luciferase activity. After treatment of the cells for 24 h with dihydrotestosterone (DHT), flutamide (FLU), validate the effectiveness and specificity, also test the effect of 2,4-DDT and methoxychlor (METH). DHT is a potent agonist, 1 x 10(-10) mol/L DHT could result in the enhancement of luciferase activity, FLU is a complete antagonist, that confirmed the effectiveness and specificity of test system. 2,4-DDT and METH are partial antagonists. 3 x 10(-7) mol/L and above 2,4-DDT and METH could inhibit the androgenic activity of DHT. The assay we developed is doful, 2,4-DDT and METH could antagonize the androgenic effects.