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Molecular characterization of Egyptian Trichomonas vaginalis clinical isolates by HSP70 restriction fragment length polymorphism

Hussien, E.M.; El-Sayed, H.Z.; El-Moamly, A.A.; Helmy, M.M.; Shaban, M.M.

Journal of the Egyptian Society of Parasitology 35(2): 699-710

2005

ISSN/ISBN: 1110-0583
PMID: 16083077
Document Number: 588653
Molecular typing of 20 Egyptian Trichomonas vaginalis clinical isolates was performed using the Restriction Fragment Length Polymorphism (RFLP) analysis employing a probe from the heat.-inducible cytoplasmic HSP70 gene family hybridized with EcoR I-digested genomic DNA. In each of the isolates tested 5 to 6 distinct DNA fragments ranging from 2.7 Kb to 7.5 Kb in size were detected. Analysis of 13 isolates from symptomatic and 7 isolates from asymptomatic women revealed 6 distinct RFLP pattern subtypes of T. vaginalis. Eleven isolates (55%) showed the same RFLP pattern, teen of them (90.9%) were from symptomatic patients. T. vaginalis Virus (TVV) was present in 7 isolates (35%). Only one isolate was considered resistant to Metronidazole. There were no relations between TVV infection or Metronidazole susceptibility and RFLP subtypes.

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