Cryopreservation and abiotic stress tolerance in potato: a proteomic approach
Criel, B.; Panta, A.; Carpentier, S.; Renaut, J.; Swennen, R.; Panis, B.; Hausman, J.F.
Communications in Agricultural and Applied Biological Sciences 70(2): 83-86
2005
ISSN/ISBN: 1379-1176 PMID: 16366280 Document Number: 588240
The effects of a vitrifying PVS2 solution (containing 30% glycerol, 15% ethylene glycol, 15% DMSO and 0.4 M sucrose) on the regeneration of shoot tips of 4 potato cultivars (Desiree, Pinaza, Wila Yari and Compis) subjected to freezing using the straw (meristems, together with the PVS2 solution, were drawn into a straw that was placed in a syringe, which was then sealed and plunged into liquid nitrogen) or aluminium foil (a drop of the PVS2 solution containing meristems was placed onto a strip of aluminium foil that was plunged into liquid nitrogen) method were studied. Shoot tips were exposed to PVS2 for 0, 10, 20, 30, 40, 50 60 or 70 minutes. The control treatment showed a decline in post-thaw survival rate with increasing duration of PVS2 treatment. For Desiree, Pinaza and Compis, the optimum post-thaw survival rates were obtained after 40-50 minutes of exposure to PVS2. Tolerance of cryopreservation significantly varied among the cultivars. Desiree, Pinaza and Compis resulted in higher post-thaw survival rates than Wila Yari, which exhibited post-thaw survival only when the aluminium foil method was used (although at very low rates). Protein extraction based on phenol extraction/ammonium acetate-methanol precipitation was conducted, and purified proteins were separated on 24-cm IPG strips. The average number of protein spots was 1210+or-33.