Understanding the response of dendritic cells to activation by streptococcal preparation OK-432
Kanzaki, N.; Terashima, M.; Kashimura, S.; Hoshino, M.; Ohtani, S.; Matsuyama, S.; Hoshino, Y.; Kogure, M.; Oshibe, I.; Endo, H.; Saito, T.; Yaginuma, H.; Gotoh, M.; Ohto, H.
Anticancer Research 25(6b): 4231-4238
2005
ISSN/ISBN: 0250-7005 PMID: 16309221 Document Number: 587872
Background: The streptococcal preparation OK432 induces maturation of T cells and dendritic cells (DCs). However, the mechanisms by which OK-432 induces DC maturation are not well understood.Materials and Methods: The effects of OK-432 and TNF-alpha on peripheral blood mononuclear cells (PBMCs) were compared. Antibody-based approaches were used to detect proteins characteristic of antigen-presenting cells and cytokines. Cytotoxicity was evaluated by measuring the release of LDH after incubation of effector and target cells. The TLR-4 levels were measured by real-time quantitative PCR.Results: Changes in cell surface marker levels were detected in both treatment groups but OK-432 had a greater effect on induction of Th-1-type cytokines. Furthermore, TLR-4 mRNA was up-regulated in cells from two out of five patients in response to OK-432, but not in response to TNF-alpha.Conclusion: OK-432 has a more profound effect on human DCs than TNF-alpha and may act through multiple signaling pathways.