Association of XRCC1 polymorphisms and chromosomal damage levels in coke-oven workers
Leng, S-Guang.; Cheng, J.; Zhang, L-Yuan.; Pan, Z-Fei.; Dai, Y-Fei.; Niu, Y.; Sun, Y-Feng.; Li, B.; He, F-Sheng.; Zheng, Y-Xin.
Zhonghua Yu Fang Yi Xue Za Zhi 39(3): 159-163
2005
ISSN/ISBN: 0253-9624 PMID: 15938843 Document Number: 587676
Objective To investigate the association of XRCC1 polymorphisms and chromosomal damage levels in peripheral blood lymphocyte in coke-oven workers. Methods The study included 141 coke-oven workers who exposed to a high level of polycyclic aromatic hydrocarbon and 66 non-exposed controls. Urinary 1-hydroxypyrene and chromosome damage in peripheral lymphocyte were measured. Four haplotpe-tagging single nucleotide polymorphisms in XRCC1 gene, including C26304T, G27466A, G28152A and G36189A, were detected and the XRCC1 haplotypes were estimated by using an extension of Clark algorithm. The associations between haplotype pairs and micronuclei data were assessed by analysis of covariance in the exposed and non-exposed groups. Results The geometric means of urinary 1-hydroxypyrene levels in coke-oven workers and the controls were 12.0 and 0.7 mu mol/mol Cr respectively (P < 0.01). The cytokinesis-block micronucleus cytokinesis-block micronucleus frequencies ( number of micronucleus per 1 000 binucleated lymphocytes) was significantly higher in coke-oven workers (0.95 +/- 0.66)% than in the controls (0.40 +/- 0.36)%, P < 0.01. The haplotype CGGG was associated with the decreased frequencies of total micronucleus, and the haplotypes TGCG (P = 0.01) and CGAG (P < 0.05) were associated with the increased frequencies of total micronucleus in the multivariate analysis with adjustment for covariates among coke-oven workers. Conclusions The genetic polymorphisms of XRCC1 gene could influence the chromosome damage levels in coke-oven workers.