Quantitative and functional assay of MDR1/P170-mediated MDR in ascites cells of patients with ovarian cancer
Krasznai, Zárd.Tibor.; Friedländer, E.; Nagy, Aás.; Szabó, Gábor.; Vereb, Görgy.; Goda, K.; Hernádi, Zán.
Anticancer Research 25(2a): 1187-1192
2005
ISSN/ISBN: 0250-7005 PMID: 15868961 Document Number: 587353
Background: MDR1-associated P-glycoprotein-dependent multidrug resistance is a common cause of chemotherapy failure in patients with advanced ovarian cancer. Here, we describe a clinical method for simultaneously assessing the expression and function of the MDR1/Pgp in tumour cells from ascites of patients with malignant ovarian carcinoma. Materials and Methods: Cells from ascites from 35 patients were collected. The expression and function of Pgp were detected by flow cytometry. For functional study, rhodamine 123 was used. Results: Using the Pgp- specific UIC2 and MM6.15 antibodies, we demonstrated the presence of Pgp in 10-79% (38.9 +/- 20, 7; n=35) of the CA 125 positive cell subpopulations. The results of the functional assay showed strong correlation with the level of Pgp expression (r=0.976; p=3.2x10(-5)). Conclusion: Direct detection of the expression level and function of MDR1/Pgp in the ascites provide useful information for the more efficient treatment of malignant diseases by proper adjustment of the chemotherapeutic protocol.