Protection of esophageal multi-lineage progenitors of squamous epithelium (stem cells) from ionizing irradiation by manganese superoxide dismutase-plasmid/liposome (MnSOD-PL) gene therapy
Niu, Y.; Shen, H.; Epperly, M.; Zhang, X.; Nie, S.; Cao, S.; Greenberger, J.S.
In Vivo 19(6): 965-974
2005
ISSN/ISBN: 0258-851X PMID: 16277008 Document Number: 586616
Background: Intraesophageal manganese superoxide dismutase plasmid liposome (MnSOD-PL) gene therapy protects against irradiation damage. Materials and Methods: To determine whether esophageal side population (SP) stem cells were protected, epitope-tagged (hemagglutinin) (I-M) MnSOD-PL was administered to C57BL/6J mice 24 hours prior to 30 Gy esophageal irradiation. SP cells were isolated, and apoptosis and multi-lineage vimentin/endothelin/F4/80 (macrophage) colonies in vitro were quantitated. Results: The number and percent of SP cells, apoptotic cells, or numbers of multi-lineage vimentin/endothelin/F4/80-positive in vitro colonies isolated from non-irradiated HA-MnSOD-PL-treated or 30 Gy-irradiated esophagus did not differ between groups. Irradiation in vitro significantly increased apoptosis in explanted non-SP cells from control (p = 0.021) compared to MnSOD-PL-treated mice. Irradiation-induced cell division was significantly increased in SP cells from control-irradiated mice (p = 0.001), but not MnSOD-PL-treated mice. Irradiation-induced apoptosis detected in vivo at 5 days was decreased by MnSOD-PL. Conclusion: MnSOD-PL gene therapy protects esophageal SP cells from irradiation in vitro and in vivo.