Recommended method for recovery of Toxocara and other geohelminth eggs from soil
Mizgajska-Wiktor, H.
Wiadomosci Parazytologiczne 51(1): 21-22
2005
ISSN/ISBN: 0043-5163 PMID: 16841685 Document Number: 586282
The flotation method elaborated for recovery of Toxocara and other geohelminth eggs from soil is described. Soil samples of about 500 ml volume are picked from 3-cm superficial layer of the ground. In the laboratory, 40 g of dry and sifted material is analysed according to following procedure: 1 h standing, 20 minutes shaking and 3 minutes centrifugation (1500 rpm) in 5% sodium hydroxide (NaOH), then centrifugation, like above, with H2O for washing the sample and next with the saturated sodium nitrate (NaNO3) for flotation the eggs. Specimen is prepared by placing a cover slip on the positive meniscus of the flotation liquid.