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Rapid discrimination between strains of beta haemolytic streptococci by intact cell mass spectrometry

Kumar, M.Palani.; Vairamani, M.; Raju, R.Prasada.; Lobo, C.; Anbumani, N.; Kumar, C.P.Girish.; Menon, T.; Shanmugasundaram, S.

Indian Journal of Medical Research 119(6): 283-288

2004

ISSN/ISBN: 0019-5340
PMID: 15243166
Document Number: 583240
Background & objectives: Beta haemolytic streptococci belonging to Lancefield group A, B, C and G cause a wide spectrum of clinical diseases. Hence there is a need for rapid and accurate typing of these strains. The present study was undertaken to evaluate the use of intact cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for rapid discrimination between strains of beta haemolytic streptococci. Methods: Colonies of beta haemolytic streptococci were emulsified with chemical matrix on the sample slide, dried and analyzed by MALDI-TOF-MS. Results: The reproducibility of results for all groups of beta haemolytic streptococci was good and spectra obtained for Lancefield group A, C and G streptococci showed discrimination between the groups on visual comparison. A finer difference in spectrum was observed among group A streptococci isolated from different locations at different periods of time. Interpretation & conclusion: MALDI-TOF-MS may be a potential tool in discriminating between strains of beta haemolytic streptococci, and also in the characterisation of untypable strains of group A streptococci.

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