Application of particular human fecal microbiota profile for disease precaution
Benno, Y.
Rinsho Byori. Japanese Journal of Clinical Pathology 52(5): 440-447
2004
ISSN/ISBN: 0047-1860 PMID: 15206132 Document Number: 582868
By a sequence analysis of 16S rDNA sequences libraries, the whole structure of human fecal microbiota has emerged. Among a total of 744 clones isolated from fecal specimens of three healthy adults, approximately 25% belonged to 31 known species. Seventy-five percent of the remaining clones were novel "phylotypes". The 16S rDNA libraries included the Bacteroides group, the Streptococcus group, the Bifidobacterium group, and the Clostridium rRNA clusters IV, IX, XIVa, and XVIII. From the results, marked individual differences in the composition of human fecal microbiota were found. Terminal restriction fragment length polymorphism (T-RFLP) analysis was used to characterize and compare human fecal microbiota among individuals. By T-RFLP, the differential profiles of fecal microbiota among individuals were noted. The species composition of fecal bifidobacteria among individuals was stable. Molecular analysis of fecal microbiota could be used for disease precaution.