Cytokines and cytokine therapy in gastrointestinal diseases
Tsaregorodtseva, T.M.; Serova, T.I.; Il'ichenko, L.Iu.; Sukhareva, G.V.; Sokolova, G.N.; Trubitsyna, I.E.; Nikol'skaia, K.a.; Klishina, M.V.; Lazebnik, L.B.
Terapevticheskii Arkhiv 76(4): 69-72
2004
ISSN/ISBN: 0040-3660 PMID: 15174327 Document Number: 582548
Aim. To compare cytokine status in gastrointestinal diseases (GID) with reference to etiological factor, course, stage, therapy of the disease. Material and methods. Enzyme immunoassay was used to examine cytokines in the peripheral blood, tissue homogenates of 560 GID patients. GID were represented by ulcer disease (UD), cholelithiasis, chronic hepatitis (CH), glutenic enteropathy (GE), Crohn's disease (CD), nonspecific ulcer colitis (NUC). Results. In chronic recurrent GID (UD, cholelithiasis, GE) ear v exacerbation was characterized by elevated concentrations of IL-lbeta IL-6, IL-8. Concentrations of IL-12, Infalpha,g, TNFalpha reached maximum on the height of the disease. Intensification of regenerative processes raised concentrations of IL-4, IL-10. An overall level of serum cytokines averaged 190-780 pg/ml, reaching in some patients with active disease 1200-3000 pg/ml, in remission 30-110 pg/ml, in the control 40 pg/ml. In chronic progressive GID the levels of IL-1beta, IL-2, IL-8, IL-6 reached 30-80 pg/ml, IL-12, Infgamma, TNFalpha-150-370 pg/ml. A rise in cytokines concentrations in inflammatory viral, bacterial, autoimmune GID was higher than in cancer, alcoholism-related diseases, metabolic disturbances. Basic therapy in patients with chronic recurrent GID led to a significant fall in concentration of serum cytokines. Therapy with monoclonal antibodies to TNFalpha was associated with transitory pronounced favourable changes in peripheral blood cytokine status. Conclusion. GID provoke elevation of serum and tissue cytokines, impairment of cytokine balance in correlation with the etiological factor, variants of the course, stage of the disease, on-going therapy.