The NMR spin-echo method is used for measurements of the translational water diffusion selectively along the apoplast and the vacuolar and cytoplasmic symplasts of plant tissue
Anisimov, A.V.; Ionenko, I.F.; Romanov, A.V.
Biofizika 49(5): 891-896
2004
ISSN/ISBN: 0006-3029 PMID: 15526476 Document Number: 575908
Simple methods for the registration of translational diffusion of water in apoplast and vacuolar and cytoplasmic symplasts were developed. The methods are based on spin-echo NMR with a pulsed magnetic field gradient and are realized by preliminary inversion of magnetization and the use of paramagnetic doping. It was shown that the diffusion of a part of root water in segments of mais roots is more enhanced than that for the bulk water. The results are explained by the appearance of the rotational movement of the protoplasm and in terms of the hypothesis of water transfer along the apoplast, which balances the cytoplasmic symplast.