Fusion expression of Neisseria gonorrhoeae outmembrane protein with a mucosal adjuvant
Pan, J.; Mao, X.; Zhang, W.; Lu, D.; Luo, P.; Wang, N.
Zhonghua Nan Ke Xue 10(4): 269-271; 274
2004
ISSN/ISBN: 1009-3591 PMID: 15148922 Document Number: 571103
To express a fusion protein of Neisseria gonorrhoeae with a mucosal adjuvant. The gene coding Loop VI-VIII(PL678) of porin, an out-membrane protein of Neisseria gonorrhoeae, was obtained by PCR. It was inserted into a plasmid fused with subunit B of heat labile enterotoxin. The recombinant was transformed in E. coli. The expression of fusion protein was analysed by ELISA, SDS-PAGE and Western-blot. Fusion protein with LTB was successfully expressed, and displayed both the ability of binding GM1 and the reactogenicity with polyclonal antibodies against Neisseria gonorrhoeae. The expression of fusion protein laid a foundation for the study of the intramolecular vaccine against Neisseria gonorrhoeae.