Absence of BRAF gene mutations differentiates spitz nevi from malignant melanoma
Mihic-Probst, D.; Perren, A.; Schmid, S.; Saremaslani, P.; Komminoth, P.; Heitz, P.U.
Anticancer Research 24(4): 2415-2418
2004
ISSN/ISBN: 0250-7005 PMID: 15330192 Document Number: 569983
Background: Distinction of Spitz nevus from malignant melanoma is sometimes difficult on the basis of conventional histology. A high rate of BRAF gene mutations in malignant melanomas (66%) and nevi (82%) has recently been reported. Materials and Methods: We screened a series of 20 Spitz nevi for BRAF mutations in exons 11 and 15 by denaturing gradient gel electrophoresis (DGGE). Results: BRAF mutations could not be identified in Spitz nevi. Conclusion: Our results show that mutations within the BRAF gene are useful markers for the differential diagnosis between Spitz nevus and malignant melanoma.