Putative mechanism of experimental immunological liver injury and influence of iron low-load on the injury in rat
Zhuang, J.; Wang, Z.; Liu, J.; Gou, L.
Wei Sheng Yan Jiu 33(6): 687-689
2004
ISSN/ISBN: 1000-8020 PMID: 15727178 Document Number: 569375
To investigate the effects of superoxide dismutase (SOD) and reducing iron load on experimental immunological liver injury in rat. 48 male Wistar rats were divided into six groups randomly. The animal model of iron low-load was created by intravasation of deferoxamine (DFO) or phlebotomy respectively. Immunological liver damage model was reproduced by injection of lipopolysaccharide (LPS) and BCG (Bacilli Calmette Guerin). The following parameters were determined such as serum iron (SI), transferrin (TRF) concentration, total proteins (TP) volume, the serum activities of aspartate aminotransferase (AST), and malondialdehyde (MDA) content, the activities of superoxide dismutase and iron deposition (HIC) in liver tissue extracts. In comparison with blank control group, the SI levels lowered (P < 0.05) in pure DFO group, pure phlebotomy group and liver injury group. Compared with each own control groups, the serum activities of AST in all injury groups augmented and the levels of TP in them reduced significantly. The increased magnitude of the activities of AST and the decreased magnitude of TP levels in DFO injury group and phlebotomy injury group were less than those of pure injury group (P < 0.05). In comparison with the control group, the MDA levels in pure DFO and phlebotomy groups declined significantly and the activities of SOD in them had no significant difference. Compared with each own control groups, the MDA levels augmented and the activities of SOD reduced significantly in all injury groups. SOD activity reduces in immunological liver injury in rat. Iron low-load can mitigate the severity of liver injury.