Determination of fleroxacin in pure and tablet forms by liquid chromatography and derivative UV-spectrophotometry
Kowalczuk, D.; Hopkała, H.
Journal - Association of Official Analytical Chemists 86(2): 229-235
2003
ISSN/ISBN: 0004-5756 PMID: 12723910 Document Number: 564076
Derivative UV-spectrophotometric and liquid chromatographic (LC) methods for fleroxacin determination were validated. In the spectrophotometric assay, first-, second-, third-, and fourth-order measurements were applied with the use of peak-zero and peak-peak techniques. The linear correlation between amplitude of the peak and concentration of the examined drug ranged from 2.0 to 12.0 micro/mL. An isocratic LC analysis was performed on a Purospher ODS column with an acidic mobile phase containing tetrabutylammonium hydroxide. Measurements were made at a wavelength of 285 nm with 4-aminobenzoic acid (PABA) as internal standard. The calibration curve was linear (r = 0.9999) in the studied range of concentration (1.0-10.0 microg/mL). The accuracy (mean recovery, about 100%), precision (relative standard deviation < 1%), selectivity, and sensitivity of the elaborated methods were satisfactory.