Kinetic study of derivatives of phenylcarbamic acid enantiomers in rabbit blood serum using an on-line coupled column liquid chromatographic system
Rojkovicová, T.; Lehotay, J.; Cizmárik, J.
Die Pharmazie 58(7): 483-486
2003
ISSN/ISBN: 0031-7144 PMID: 12889532 Document Number: 563544
An on-line coupled HPLC system is described for the kinetic study of the enantiomers of 1-methyl-2-piperidinoethylesters of 2-, 3- and 4-alkoxyphenylcarbamic acid in rabbit blood serum. The method involves three steps: (i) off-line pre-concentration and cleanup step, (ii) analytical separation of the racemate on a reversed-phase stationary phase, and (iii) separation of the enantiomers on a teicoplanin aglycone chiral stationary phase (Chirobiotic Tag). The limit of the determination with standard solutions was approximately 5.0 microg/ml. In vitro degradation studies of enantiomers have demonstrated differences in the concentration of the enantiomers after the treatment. The rate constants of R(-)- and S(+)-forms of enantiomers (the same as the position of alkoxychain) were, not significantly different. The number of carbon atoms had an influence on the degradation kinetics.