Top Abstract Purchase Related
Home
>
Node
>
Subnode

Detection of genotoxicity of atmospheric particles using a high-throughput microplate umu-test system

Funasaka, K.; Kitano, M.; Nakama, A.; Yoshikura, T.; Oda, Y.

Acta Biochimica Polonica 50(1): 291-296

2003

ISSN/ISBN: 0001-527X
PMID: 12673371
Document Number: 563076
A previously developed and highly sensitive umu-microplate test system based on the nitroreductase- and O-acetyltransferase-overproducing strain Salmonella typhimurium NM3009 and the O-acetyltransferase-overproducing strain S. typhimurium NM2009 was applied to the detection of genotoxic activity in atmospheric particles in urban areas using a relatively small sample load. The results showed that the test system was able to detect slight increases in induced genotoxicity in atmospheric particles and that genotoxicity was detected mainly in the fine fraction but also partially in the coarse fraction. The present sensitive microplate test system has potential for application to the screening of various other environmental samples.

Document emailed within 1 workday
Secure & encrypted payments

Eisentraeger, A.; Brinkmann, C.; Michel, K.; Hahn, S.; Huettner, M.; Weber, G. 2004: Development of automated high-throughput ecotoxicity and genotoxicity test systems and fields of application Water Science and Technology: a Journal of the International Association on Water Pollution Research 50(5): 109-114
Huo, J.; Liu, Y.; Zeng, Z.; Zhu, X.; Peng, Z.; Chen, J.; Zhang, L. 2019: Assessment of the genotoxicity of 2-methylfuran based on a multi-endpoint genotoxicity test system in vivo Wei Sheng Yan Jiu 48(6): 976-1000
Dugan, A.M.; Barilyak, I.R. 1995: The evaluation of the mutagenic activity of gaseous particles in the atmospheric air by using a microbiological test Mikrobiolohichnyi Zhurnal 57(4): 66-72
Li, Y.-Z.; Chen, Z.-Y.; Wang, H.; Huang, H.; Song, Q.-X.; Zhou, G.-H. 2012: Establishment of a hydrogel chip for high-throughput detection of y chromosome microdeletions Zhonghua Nan Ke Xue 18(2): 109-114
Devine, P.L. 1992: A sensitive microplate assay for glycoproteins that utilizes an immunological digoxigenin-based detection system Biotechniques 12(2): 160; 162
Jouquand, S.; André, C.; Chéron, A.; Hitte, C.; Chuat, J.C.; Galibert, F. 2000: Using the fluorogenic 5' nuclease assay for high-throughput detection of (CA)n repeats in radiation hybrid mapping Biotechniques 28(4): 754-8 760-2 764-5
Sasaki, N. 1997: Development of high-throughput polymerase chain reaction system and its performance Hokkaido Journal of Medical Science 72(3): 249-259
Jena, G.B.; Bhunya, S.P. 1992: Thirty day genotoxicity study of an organophosphate insecticide, monocrotophos, in a chick in vivo test system In Vivo 6(5): 527-530
Andrus, A.; Wright, P.; Wang, J.; Mullah, B.; Baier, J.; Mason, J.; Kaufman, G. 1995: Synthesis and purification in a single column on a high-throughput automated oligonucleotide production system Nucleic Acids Symposium Series 34: 183-184
Gelderblom, H.C.r.; Menting, S.; Beld, M.G. 2006: Clinical performance of the new rRoche COBAS TaqMan HCV Test and High Pure System for extraction, detection and quantitation of HCV RNA in plasma and serum Antiviral Therapy 11(1): 95-103
Lei, X.F.; Xing, Q. 1993: The genotoxicity of air particles tested by SOS chromotest Zhonghua Yu Fang Yi Xue Za Zhi 27(3): 141-143
Stefan, P.; Moritz, E.; Alexander, W.; Matthias, W. M.; Norbert, D.; Annette, W.; Thomas, F.; Pfützner Andreas 2009: Development of a High-Throughput Method for Screening the Dopamine D2 (DRD2) Receptor Gene Polymorphisms Based on the LightCycler® System Clinical Laboratory 55(9-10): 353-358
Itoh, Y. 1979: Absorption test using latex particles as the indicator system for the species identification of bloodstains and muscles Journal of Forensic Sciences 24(3): 561-568
Mosqueron, L.; Le Moullec, Y.; Momas, I. 2001: Assessment of exposure to atmospheric particles: contribution of individual measurements Revue d'Epidemiologie et de Sante Publique 49(1): 77-91
Vlase, L.; Popa, D.-S.; Muntean, D.; Mihu, D.; Leucuta, S. 2009: A new, high-throughput high-performance liquid chromatographic/mass spectrometric assay for therapeutic level monitoring of digoxin in human plasma Journal - Association of Official Analytical Chemists 92(5): 1390-1395
Winter, G.F. 1985: Rubella antibody detection by a microplate haemolysis-in-gel technique Medical Laboratory Sciences 42(1): 56-59
Alessio, L.; Cambiaghi, G.; Croce, E.; Frigieri, P.; Trucco, R. 1979: Determination of lead, cadmium, zinc and iron in atmospheric particles in industrialized urban areas in Lombardy La Medicina del Lavoro 70(1): 24-37
Tansuphasiri, U.; Suttirat, S. 2001: Simple microplate hybridization assay for detection of amplified products of Mycobacterium tuberculosis Southeast Asian Journal of Tropical Medicine and Public Health 32(4): 835-843
Gimeno, R.A.; Altelaar, A.F.M.; Marcé, R.M.; Borrull, F. 2002: Determination of polycyclic aromatic hydrocarbons and polycylic aromatic sulfur heterocycles by high-performance liquid chromatography with fluorescence and atmospheric pressure chemical ionization mass spectrometry detection in seawater and sediment samples Journal of Chromatography A 958(1-2): 141-148
Hongo, T.; Mizuno, Y.; Haraguchi, S.; Yoshida, T.O. 1986: A new anticancer drug sensitivity test using the microplate culture and surviving tumor cell staining method Gan to Kagaku Ryoho. Cancer and ChemoTherapy 13(2): 247-254