Determination of adenosine, rutin and quercetin in Carthamus tinctorius by HPCE
Sun, Y.; Guo, T.; Sui, Y.; Li, F-mei.
Yao Xue Xue Bao 38(4): 283-285
2003
ISSN/ISBN: 0513-4870 PMID: 12889129 Document Number: 561830
A high performance capillary electrophoresis method was developed for the determination of adenosine, rutin and quercetin in C. tinctorius. A fused silica capillary (66.5 cm x 50 micro m ID, an effective length of 58 cm) was used. The running buffer was composed of 50 mmol borax/litre (pH 9.7) containing 18% methanol. The applied voltage was 24 kV and the capillary temperature was 20 degrees C. The detection wavelength was 210 nm. Rifampicin was used as the internal standard. A good linearity between peak area ratio of the common peak to the internal standard and the concentration was found in the range of 10-160 mg/litre for adenosine, 10-2000 mg/litre for rutin and 100-1600 mg/litre for quercetin (r<0.998). The average recoveries were 98.5-100.5%, 96.9-99.5% and 99.1-99.5% for adenosine, rutin and quercetin, respectively. The relative standard deviation was less than 6.5% (n=5). The method was simple and rapid, with satisfactory recoveries and good reproducibilities, and can be used to control the quality of C. tinctorius.