Disruption of basement membrane, extracellular matrix metalloproteinases and E-cadherin in renal-cell carcinoma
Morell-Quadreny, L.; Rubio, J.; Lopez-Guerrero, J.A.; Casanova, J.; Ramos, D.; Iborra, I.; Solsona, E.; Llombart-Bosch, A.
Anticancer Research 23(6d): 5005-5010
2003
ISSN/ISBN: 0250-7005 PMID: 14981959 Document Number: 558757
A retrospective study was performed to determine the prognostic value of Basement Membrane (BM) integrity, Matrix Metalloproteinases (MMPs) and E-Cadherin expression in renal cell carcinoma (RCC). An immunohistochemical study on laminin and collagen IV, MMPs 1 and 2, and E-Cadherin was carried out on 71 RCCs. BM fragmentation was considered taking 75% as a cut-off. MMP 1 and MMP2 immunostaining, as well as E-Cadherin was considered taking 25% as a cut-off. An inverse relationship was seen between E-Cadherin with laminin, collagen IV and MMPs. More than 75% loss of laminin, collagen IV and E-Cadherin, as well as higher expression of MMPs, were associated with symptoms, tumoral size and worse grade. Loss of collagen IV and E-Cadherin were of prognostic value. Both BM and E-Cadherin are good prognostic markers. MMPs patterns show a relationship between BM proteins and E-Cadherin, but evaluation is more time-consuming and provide no better prognostication; consequently they are not useful in routine clinical applications.