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Photodynamic inactivation of porphyrin sensitized T7 phages: efficiency and mechanism of action

Egyeki, M.; Turóczy, G.; Tóth, K.; Fekete, A.; Maillard, P.; Csík, G.

Acta Pharmaceutica Hungarica 73(2): 97-102

2003

ISSN/ISBN: 0001-6659
PMID: 14702690
Document Number: 557390
We investigated the efficiency and the mechanism of action of two glycoconjugated tetraphenyl porphyrins in their photoreaction with T7 bacteriophage. Both types of porphyrins sensitized the photoinactivation of T7, but the slopes of inactivation kinetics were markedly different. Our result suggests that both type I and type II reaction play a role in the virus inactivation. Optical melting studies revealed structural changes in the protein part but not in the DNA of the photo-chemically treated nucleoprotein complex. Polymerase chain reaction (PCR) analysis failed to demonstrate any DNA damage.

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Allison, R.R.; Sibata, C.H. 2010: Photodynamic therapy: mechanism of action and role in the treatment of skin disease Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale Societa Italiana di Dermatologia e Sifilografia 145(4): 491-507
Godal, A.; Nilsen, N.O.; Klaveness, J.; Branden, J.Erik.; Nesland, J.M.; Peng, Q. 2006: New derivatives of 5-aminolevulinic acid for photodynamic therapy: chemical synthesis and porphyrin production in in vitro and in vivo biological systems Journal of Environmental Pathology Toxicology and Oncology: Official Organ of the International Society for Environmental Toxicology and Cancer 25(1-2): 109-126
Barabash, R.D.; Normanskiĭ, V.E.; Andreeva, K.P.; Galil-Ogly, G.A.; Kolobanov, A.S. 1989: Pathogenesis of porphyrin-sensitized lethal and sub-lethal photo-injury Biulleten' Eksperimental'noi Biologii i Meditsiny 108(10): 435-437
Piette, J.; Calberg-Bacq, C.M.; Van de Vorst, A. 1978: Photodynamic activity of dyes with different DNA binding properties II. T4 phage inactivation International Journal of Radiation Biology and Related Studies in Physics Chemistry and Medicine 34(3): 223-232
Mac Knight, M.L.; Spikes, J.D. 1970: The time-course of the destruction of amino acid residues during the photodynamic inactivation of ribonuclease a Bollettino Chimico Farmaceutico 109(11): 659-664
Kholodiĭ, G.I.; Mindlin, S.Z. 1988: Rpo pathway as a possible recombination mechanism of the interaction between DNA of transducing phages and the Escherichia coli genome Genetika 24(10): 1739-1751
Krüger, D.H.; Presber, W.; Mann, W.; Rosenthal, H.A. 1972: Studies of T3 phages. 3. Effect of various nutrient deficiencies on the reproduction of phages T3 and T7 Acta Biologica et Medica Germanica 29(4): 477-482
Curnow, A.; Pye, A. 2007: Biochemical manipulation via iron chelation to enhance porphyrin production from porphyrin precursors Journal of Environmental Pathology Toxicology and Oncology: Official Organ of the International Society for Environmental Toxicology and Cancer 26(2): 89-103
Schermuly, E.; Doss, M. 1976: Porphyrin biosynthesis from ALA and PBG by human erythrocytes in porphyrin disorders. Kinetic studies of the isomer series i and IIi Annals of Clinical Research 8(Suppl 17): 92-102
Shliapnikova, E.A.; Savitskiĭ, A.P.; Kachalova, G.S. 1986: Accessibility of porphyrin macrocycle in myoglobin at various pH levels. Fluorescence study of the porphyrin-globin complex Molekuliarnaia Biologiia 20(1): 138-145
Mito, K. 1996: Photodynamic efficiency of macrophage activity using a photosensitizer, lumin, with near-IR laser light for photoimmunotherapy of a cancer Frontiers of Medical and Biological Engineering: the International Journal of the Japan Society of Medical Electronics and Biological Engineering 7(2): 81-92
Knappstein, K.; Aust, V.; Kunz, H-Jürgen.; Kaske, M. 2013: Efficiency of two commercial on-farm pasteurizers for inactivation of mastitis pathogens in milk intended for feeding of calves Berliner und Munchener Tierarztliche Wochenschrift 126(1-2): 32-36
Tadakuma, T.; Yasuda, T. 1981: Analysis of the mechanism of B cell activation by using hapten-sensitized liposomal model membranes Nihon Saikingaku Zasshi. Japanese Journal of Bacteriology 36(6): 719-724
Sebti, S.M.; Jani, J.P.; Mistry, J.S.; Gorelik, E.; Lazo, J.S. 1991: Metabolic inactivation: a mechanism of human tumor resistance to bleomycin Cancer Research 51(1): 227-232
Nowak, A.; Woytoń, A.; Baran, E. 1974: Photodynamic action of extractum hyperici in the treatment of vitiligo Przeglad Dermatologiczny 61(1): 77-82
Pessah, I.N.; Durie, E.L.; Schiedt, M.J.; Zimanyi, I. 1990: Anthraquinone-sensitized Ca2+ release channel from rat cardiac sarcoplasmic reticulum: possible receptor-mediated mechanism of doxorubicin cardiomyopathy Molecular Pharmacology 37(4): 503-514
Armstrong, A.P.; Hollenberg, P.F. 1999: Mechanism-based inactivation of rat liver cytochrome P-450 2B1 by 2-methoxy-5-nitrobenzyl bromide Drug Metabolism and Disposition: the Biological Fate of Chemicals 27(6): 741-745
Van Lente, F.; McHugh, A.; Pippenger, C.E. 1986: Carbamylation of apo-aspartate aminotransferase: a possible mechanism for enzyme inactivation in uremic patients Clinical Chemistry 32(11): 2107-2108
Fu, N.W.; Chu, Y.X. 1989: Photodynamic action of hypocrellin a on hepatoma cell mitochondria and microsomes Zhongguo Yao Li Xue Bao 10(4): 371-373
Bresler, S.E.; Vasil'eva, N.N.; Kazbekov, E.N. 1976: The mechanism of action of d-amino acid oxidase. I. Evidence for a free radical mechanism of the reaction catalyzed b a dimeric form of the enzyme Molekuliarnaia Biologiia 10(2): 260-269