Mechanisms of the alkaloid aurantioclavine excretion and uptake during Penicillium nalgiovense VKM F-229 growth
Zhelifonova, V.P.; Kulakovskaia, T.V.; Kozlovskiĭ, A.G.
Mikrobiologiia 72(2): 183-188
2003
ISSN/ISBN: 0026-3656 PMID: 12751241 Document Number: 556455
The biphasic dynamics of the alkaloid aurantioclavine in the culture liquid of Penicillium nalgiovense VKM F-229 is shown to be due to the diauxic growth of the fungus on two carbon sources, succinate and mannitol. In the phase of active growth on succinate, the fungus synthesizes aurantioclavine and excretes it into the medium in an energy-independent manner, as a result of which the concentration of the alkaloid in the culture liquid rises. During the phase of metabolic adaptation to the other carbon source, mannitol, the concentration of aurantioclavine in the culture liquid falls, probably due to the energy-dependent uptake of the alkaloid by fungal cells. The reversible excretion of aurantioclavine in P. nalgiovense indicates that these are regulated processes and depend on the growth parameters and the physiological state of the fungus.