Determination of the inhibitory activity of angiotensin-converting enzyme inhibitor captopril by high performance capillary electrophoresis
Xin, Z.-h.; Ma, H.-l.; Wu, S.-y.; Dai, C.-h.
Yao Xue Xue Bao 38(11): 843-845
2003
ISSN/ISBN: 0513-4870 PMID: 14991998 Document Number: 555306
To establish a method for determinate of the inhibitory activity of angiotensin-converting enzyme inhibitor captopril by high performance capillary electrophoresis. The characteristic absorptive wavelength of hippuric acid determined by ultraviolet spectrophotometer is 228 nm. The method employed a melted capillary column, 50 mmol.L-1 phosphoric acid (pH 8.3) buffer solution, inject pressure 4.8 kPa, inject time 3 s, separation voltage 20 kV and detection wavelength 228 nm. The reactant and resultant was separated completed within 7 min. IC50 of captopril was 0.019 mumol.L-1. Captopril is a competitive inhibitor, which was proved by enzyme reaction dynamics. The method was shown to be accurate, simple and rapid and can be used for determination of the inhibitory activity of captopril.