Production of biopesticides using wastewater sludge as a raw material--effect of process parameters
Tirado Montiel, M.L.; Tyagi, R.D.; Valero, J.R.; Surampalli, R.Y.
Water Science and Technology a Journal of the International Association on Water Pollution Research 48(8): 239-246
2003
ISSN/ISBN: 0273-1223 PMID: 14682592 Document Number: 554966
Production of Bacillus thuringiensis (Bt) based biopesticide was studied using hydrolysed or raw wastewater sludge as a raw material. The sludge hydrolysis was carried out at different pH (2, 4 and 6). The sludge after hydrolysis was sterilised at 121 degrees C for 30 minutes. The effect of temperature, pH and agitation speed on growth and toxin production was also investigated. The pH in the shake flask was controlled by adding 0.1 M TRIS buffer. The progress of the biopesticide production process was monitored by measuring viable cell count (VC), spore count (SC) and entomotoxicity (Tx). The entomotoxicity was measured against spruce budworm. Control of pH substantially increased the entomotoxicity of the final product. Increased agitation speed resulted in higher VC, SC and Tx values.